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Chromatographic Techniques


The methods of analysis of certain chemicals and compounds are highly specific to that chemical or the compound. Thus it needs to have separated methods to such substances. Chromatography involves a testing sample dissolved in mobile phase which is forced through an immobilized immiscible stationary phase. A particular technique chose its two phases such that components of the sample having differing solubility in each phase. A compound which is more soluble in stationary phase will takes more time to separate from the test sample where as a less soluble compound will easily separate from the test mixture taking less time. Most common chromatographic techniques that we are use in daily applications have made in this sense. So that this makes easier for us to analyze very important cases such as antibody testing in medicine, pigment separations, detecting pesticides, caring out Cladosporium bioassay 9 will be discussed latter), testing water for pollution, detecting explosives and separating amino acids etc.

Some of the most common techniques in the chromatography will be discussed here.
  1. Opencolumn chromatography.
  2. Paper chromatography
  3. Thin layer chromatography
  4. High performance liquid chromatography
  5. Gas chromatography
  6. Reversed phase chromatography
  7. Pyrolysis gas chromatography mass spectrometry
  8. Counter current chromatography
  9. Chiral chromatography



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